Modulation of Rho - dependent transcription termination in Escherichia coli by the H - NS 5 family of proteins 6 7 8 Running title : H - NS and Rho - dependent termination
نویسنده
چکیده
Submitted to: Journal of Bacteriology 1 Section: Genetics and Molecular Biology 2 3 4 Modulation of Rho-dependent transcription termination in Escherichia coli by the H-NS 5 family of proteins 6 7 8 Running title : H-NS and Rho-dependent termination 9 10 11 Shivalika Saxena and J. Gowrishankar * 12 13 Laboratory of Bacterial Genetics, Centre for DNA Fingerprinting and Diagnostics, 14 Hyderabad 500 001, India 15 16 17 * Author for correspondence 18 Mailing address : Centre for DNA Fingerprinting and Diagnostics, 19 Building 7, Gruhakalpa, 20 5-4-399/B, Nampally, 21 Hyderabad 500 001, India 22 Tel : +91-40-2474 9445 23 Fax : +91-40-2474 9448 24 Email : [email protected] 25 Copyright © 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. J. Bacteriol. doi:10.1128/JB.00220-11 JB Accepts, published online ahead of print on 20 May 2011
منابع مشابه
Modulation of Rho-dependent transcription termination in Escherichia coli by the H-NS family of proteins.
Nascent transcripts in Escherichia coli that fail to be simultaneously translated are subject to a factor-dependent mechanism of termination (also termed a polarity) that involves the proteins Rho and NusG. In this study, we found that overexpression of YdgT suppressed the polarity relief phenotypes and restored the efficiency of termination in rho or nusG mutants. YdgT and Hha belong to the H-...
متن کاملCompromised factor-dependent transcription termination in a nusA mutant of Escherichia coli: spectrum of termination efficiencies generated by perturbations of Rho, NusG, NusA, and H-NS family proteins.
The proteins NusA and NusG, which are essential for the viability of wild-type Escherichia coli, participate in various postinitiation steps of transcription including elongation, antitermination, and termination. NusG is required, along with the essential Rho protein, for factor-dependent transcription termination (also referred to as polarity), but the role of NusA is less clear, with conflic...
متن کاملBridged filaments of histone-like nucleoid structuring protein pause RNA polymerase and aid termination in bacteria
Bacterial H-NS forms nucleoprotein filaments that spread on DNA and bridge distant DNA sites. H-NS filaments co-localize with sites of Rho-dependent termination in Escherichia coli, but their direct effects on transcriptional pausing and termination are untested. In this study, we report that bridged H-NS filaments strongly increase pausing by E. coli RNA polymerase at a subset of pause sites w...
متن کاملRho and NusG suppress pervasive antisense transcription in Escherichia coli.
Despite the prevalence of antisense transcripts in bacterial transcriptomes, little is known about how their synthesis is controlled. We report that a major function of the Escherichia coli termination factor Rho and its cofactor, NusG, is suppression of ubiquitous antisense transcription genome-wide. Rho binds C-rich unstructured nascent RNA (high C/G ratio) prior to its ATP-dependent dissocia...
متن کاملRegulation of rho-dependent transcription termination by NusG is specific to the Escherichia coli elongation complex.
To terminate transcription in E. coli, Rho protein binds an RNA loading site on the nascent transcript, translocates 5'--> 3' along the RNA in an ATP-driven process, and, upon reaching the transcription elongation complex, brings about RNA release. Thus, the Rho-dependent termination process can be viewed, in part, as a kinetic competition between the rate of transcript elongation by RNA polyme...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2011